RAR alpha/NR1B1 Antibody

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Western Blot: Retinoic Acid Receptor alpha Antibody [NB100-78385] - MOLT-4 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-RARa antibody. Proteins were ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78385
    • Availability
      Product Discontinued

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RAR alpha/NR1B1 Antibody Summary

Immunogen
Recombinant (partial), N-terminal amino acids 1-100
Specificity
RAR alpha
Clonality
Polyclonal
Host
Rabbit
Gene
RARA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RAR alpha/NR1B1 Antibody

  • alpha polypeptide
  • NR1B1
  • RAR alpha
  • RARA
  • RAR-alpha
  • retinoic acid receptor, alpha

Background

RAR alpha (retinoic acid receptor alpha) is a 51 kD member of the nuclear hormone receptor family, NR1 subfamily. This nuclear protein contains modulating, DNA-binding, and steroid binding domains, and has been reported to have multiple isoforms. RAR alpha is a nuclear receptor for retinoic acid that controls cell function by regulating gene expression and inducing profound effects on vertebrate development, acting as a morphogen and powerful teratogen. RAR alpha is increased by protein kinase C stimulation, and estrogen; decreased at G0/G1 to S phase of cell cycle and in lymphocytes by cellular activation. RAR alpha can be modified by phosphorylation and has been shown to interact with NCOA3 and NCOA6 co-activators leading to strong increases in the transcription of target genes. The Poly6168 antibody recognizes the N-terminal region of human RAR alpha and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RARA
Entrez