Ran Antibody

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Western Blot: Ran Antibody [NB100-1048] - Western blot of RCC1, RanGAP1 & Ran antibodies on Hela total cell and xenopus egg extract

Product Details

Summary
Product Discontinued
View other related Ran Primary Antibodies

Order Details


    • Catalog Number
      NB100-1048
    • Availability
      Product Discontinued

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Ran Antibody Summary

Immunogen
Recombinant full length protein (Human) - His-tagged and purified from E. coli.
Localization
Nuclear
Specificity
Ran
Clonality
Polyclonal
Host
Rabbit
Gene
RAN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-1048 in the following applications:

  • WB
    1 publication

Reactivity Notes

Cross-reacts with Human and Xenopus laevis. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA and 30% Glycerol
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ran Antibody

  • Androgen receptor-associated protein 24
  • ARA24Gsp1
  • GTPase Ran
  • GTP-binding nuclear protein Ran
  • guanosine triphosphatase Ran
  • member RAS oncogene family
  • OK/SW-cl.81
  • RAN, member RAS oncogene family
  • RanGTPase
  • Ras-like protein TC4
  • Ras-related nuclear protein
  • TC4

Background

Ran is a small GTPase of the Ras superfamily that controls nucleocytoplasmic transport. However, Ran also functions in the absence of a membrane-enclosed nucleus, during cell division. RanGTP acts to promote microtubule nucleation during mitotic spindle assembly and, later during mitosis, is required for NE assembly. For correct spindle and NE assembly on chromatin, it is critical that the RanGTP concentration on or near chromatin is higher than in the surrounding mitotic cytoplasm. Unlike many other regulatory GTPases, Ran is not posttranslationally modified so that it can be targeted to the subcellular compartment where it acts. Rather, RanGTP has been thought to be localized by the differential localization of Ran regulators. During mitosis, the nucleotide exchange factor RCC1, which generates RanGTP, is in part bound to chromatin. This results in the preferential production of RanGTP on the chromatin surface. From there, RanGTP is believed to diffuse only a short distance before either encountering mediators of spindle or NE assembly or, alternatively, being reconverted to RanGDP under the influence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ran Antibody (NB100-1048)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Filter By Species
Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ran Antibody (NB100-1048). (Showing 1 - 1 of 1 FAQs).

  1. Our customer would like to purchase this antibody for IP (catalog # NB100-1048). They would like to have its concentration to estimate the titer for IP. Since it is the whole antisera product, we assume that there is no info of concentration. If so, would you please help suggest the dilution titer for IP with this antibody?
    • NB100-1048 is not purified so the exact concentration is not quantifiable. As far as the recommended dilution for IP is concerned, try 1:50 to 1:100 dilution as the starting point and if IP doesn't look very well, further optimization steps might be required.

Secondary Antibodies

 

Isotype Controls

Additional Ran Products

Bioinformatics Tool for Ran Antibody (NB100-1048)

Discover related pathways, diseases and genes to Ran Antibody (NB100-1048). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Ran Antibody (NB100-1048)

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Pathways for Ran Antibody (NB100-1048)

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PTMs for Ran Antibody (NB100-1048)

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Research Areas for Ran Antibody (NB100-1048)

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Blogs on Ran.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol RAN
Entrez
Uniprot