Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS diluted antiserum
0.1% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-1048 in the following applications:
Cross-reacts with Human and Xenopus laevis. Not yet tested in other species.
Alternate Names for Ran Antibody
Androgen receptor-associated protein 24
GTP-binding nuclear protein Ran
guanosine triphosphatase Ran
member RAS oncogene family
RAN, member RAS oncogene family
Ras-like protein TC4
Ras-related nuclear protein
Ran is a small GTPase of the Ras superfamily that controls nucleocytoplasmic transport. However, Ran also functions in the absence of a membrane-enclosed nucleus, during cell division. RanGTP acts to promote microtubule nucleation during mitotic spindle assembly and, later during mitosis, is required for NE assembly. For correct spindle and NE assembly on chromatin, it is critical that the RanGTP concentration on or near chromatin is
higher than in the surrounding mitotic cytoplasm. Unlike many other regulatory GTPases, Ran is not posttranslationally modified so that it can be targeted to the subcellular compartment where it acts. Rather, RanGTP has been thought to be localized by the differential localization of Ran regulators.
During mitosis, the nucleotide exchange factor RCC1, which generates RanGTP, is in part bound to chromatin. This results in the preferential production of RanGTP on the chromatin surface. From there, RanGTP is believed to diffuse only a short distance before either encountering mediators of spindle or NE assembly or, alternatively, being reconverted to RanGDP under the influence.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Ran Antibody (NB100-1048). (Showing 1 - 1 of 1 FAQs).
Our customer would like to purchase this antibody for IP (catalog # NB100-1048). They would like to have its concentration to estimate the titer for IP. Since it is the whole antisera product, we assume that there is no info of concentration. If so, would you please help suggest the dilution titer for IP with this antibody?
NB100-1048 is not purified so the exact concentration is not quantifiable. As far as the recommended dilution for IP is concerned, try 1:50 to 1:100 dilution as the starting point and if IP doesn't look very well, further optimization steps might be required.