Raf-1 Antibody

Images

 
Western Blot: Raf-1 Antibody [NBP1-51320] - Extracts from hela cells.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-51320] - Human brain tissue.

Product Details

Summary
Product Discontinued
View other related Raf-1 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51320
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Raf-1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 251-300 of Human Raf-1.
Specificity
Raf-1 (R282) pAb detects endogenous levels of Raf-1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
RAF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Raf-1 (R282) pAb in extracts from hela cells.
Immunohistochemistry (IHC) analyzes of Raf-1 (R282) pAb in paraffin-embedded human brain tissue.
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Raf-1 Antibody

  • cRaf
  • c-Raf
  • c-Raf-1
  • CRAFraf proto-oncogene serine/threonine protein kinase
  • EC 2.7.11
  • EC 2.7.11.1
  • NS5
  • Proto-oncogene c-RAF
  • RAF proto-oncogene serine/threonine-protein kinase
  • RAF
  • Raf1
  • Raf-1
  • Raf-1Oncogene RAF1
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • v-Raf-1

Background

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may even play the role of the messenger from membrane tyrosine kinases to Raf-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-47833
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-43792
Species: Hu
Applications: WB
H00003845-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP2-45745
Species: Hu
Applications: WB, IHC, IF
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-46403
Species: Hu
Applications: IHC
NBP1-89942
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for Raf-1 Antibody (NBP1-51320) (0)

There are no publications for Raf-1 Antibody (NBP1-51320).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Raf-1 Antibody (NBP1-51320) (0)

There are no reviews for Raf-1 Antibody (NBP1-51320). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Raf-1 Antibody (NBP1-51320) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Raf-1 Products

Bioinformatics Tool for Raf-1 Antibody (NBP1-51320)

Discover related pathways, diseases and genes to Raf-1 Antibody (NBP1-51320). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Raf-1 Antibody (NBP1-51320)

Discover more about diseases related to Raf-1 Antibody (NBP1-51320).
 

Pathways for Raf-1 Antibody (NBP1-51320)

View related products by pathway.

PTMs for Raf-1 Antibody (NBP1-51320)

Learn more about PTMs related to Raf-1 Antibody (NBP1-51320).
 

Research Areas for Raf-1 Antibody (NBP1-51320)

Find related products by research area.

Blogs on Raf-1

There are no specific blogs for Raf-1, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Raf-1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RAF1
Entrez