Raf-1 Antibody


Western Blot: Raf-1 Antibody [NBP1-51320] - Extracts from hela cells.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-51320] - Human brain tissue.

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Raf-1 Antibody Summary

Synthetic peptide, corresponding to amino acids 251-300 of Human Raf-1.
Raf-1 (R282) pAb detects endogenous levels of Raf-1 protein.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Raf-1 (R282) pAb in extracts from hela cells.
Immunohistochemistry (IHC) analyzes of Raf-1 (R282) pAb in paraffin-embedded human brain tissue.
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Raf-1 Lysate (NBP2-64908)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
15mM Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Raf-1 Antibody

  • cRaf
  • c-Raf
  • c-Raf-1
  • CRAFraf proto-oncogene serine/threonine protein kinase
  • EC 2.7.11
  • EC
  • NS5
  • Proto-oncogene c-RAF
  • RAF proto-oncogene serine/threonine-protein kinase
  • RAF
  • Raf1
  • Raf-1
  • Raf-1Oncogene RAF1
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • v-Raf-1


Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may even play the role of the messenger from membrane tyrosine kinases to Raf-1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol RAF1