Rad51D Antibody

Product Discontinued

Rad51D Antibody Summary

• Immunogen: A peptide from human Rad51D (homologue 4). [UniProt# O75771]
• Specificity: This is specific for hRAD51D. There is no cross-reactivity observed with XRCC2, XRCC3, RAD51, RAD51B, or RAD51C.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAD51D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: This Rad51D antibody is useful for Western blot, where it recognizes a band at 35 kDa representing Rad51D (H3 splice variant). Immunocytochemistry/Immunofluorescence and Immunoprecipitation were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  Rad51D Overexpression Lysate

Reactivity Notes

Human. This antibody does not work in mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad51D Antibody

• DNA repair protein RAD51 homolog 4
• HsTRAD
• R51H3Trad
• RAD51 homolog D
• RAD51DRAD51 (S. cerevisiae)-like 3
• RAD51-like 3 (S. cerevisiae)
• RAD51-like protein 3
• recombination repair protein
• TRAD

Background

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the other four, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C. RAD51D's expression pattern and sequence similarity to other RAD51 family members most likely makes it part of a complex of proteins required for DNA repair and meiotic recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad51D Antibody (NB100-166)(7)

Publications using NB100-166

• Park JY, Virts EL, Jankowska A et al. Complementation of hypersensitivity to DNA interstrand crosslinking agents demonstrates that XRCC2 is a Fanconi anaemia gene. J. Med. Genet. 2016-05-20 [PMID: 27208205] (WB, Human)
• Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013-12-01 [PMID: 24088786] (WB, Human)
• Chun J, Buechelmaier ES, Powell SN. Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway. Mol Cell Biol 2013-01-01 [PMID: 23149936] (Human)
• Wang Y, Huang JW, Calses P et al. MiR-96 Downregulates REV1 and RAD51 to Promote Cellular Sensitivity to Cisplatin and PARP Inhibition Cancer Res 2012-08-15 [PMID: 22761336] (WB, Human)
• Shammas MA, Shmookler Reis RJ, Koley H et al. Dysfunctional homologous recombination mediates genomic instability progression in myeloma. Blood 2009;113(10):2290-2297 [PMID: 19050310] (ICC/IF, Human)
• Chattopadhyay R, Das S, Maiti AK et al. Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1. Mol Cell Biol;28(23):7066-80. 2008-12-01 [PMID: 18809583]
• Wiese, C et al. Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination. Nucleic Acids Res;34(9):2833-43. 2006-05-22 [PMID: 16717288] (WB, IP, Human)

Bioinformatics

• Gene Symbol: RAD51D