RAD18 Antibody

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Immunoprecipitation: RAD18 Antibody [NB100-61062] - Detection of Human RAD18 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

RAD18 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human E3 ubiquitin-protein ligase RAD18 using the numbering given in entry NP_064550.2 (GeneID 56852).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAD18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 5 ug/mg lysate
Positive Control
RAD18 Lysate (NBL1-15113)
Publications
Read Publication using NB100-61062.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAD18 Antibody

  • EC 6.3.2.-
  • hHR18
  • hRAD18
  • postreplication repair protein hRAD18p
  • Postreplication repair protein RAD18
  • RAD18 homolog (S. cerevisiae)
  • RAD18, S. cerevisiae, homolog
  • RING finger protein 73
  • RNF73E3 ubiquitin-protein ligase RAD18

Background

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAD18 Antibody (NB100-61062)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAD18
Entrez
Uniprot