Rac1 Overexpression Lysate (Native)

Images

 
Western Blot: Rac1 Overexpression Lysate (Adult Normal) [NBL1-15104] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rac1.

Product Details

Summary
Product Discontinued
View other related Rac1 Lysates

Order Details


    • Catalog Number
      NBL1-15104
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Rac1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223632 and based on accession number NM_198829. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) (RAC1), transcript variant Rac1c, mRNA.
Gene
RAC1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Rac1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Rac1 Overexpression Lysate (Native)

  • Cell migration-inducing gene 5 protein
  • MGC111543
  • p21-Rac1
  • p21-Rac1migration-inducing gene 5
  • Rac1
  • Ras-like protein TC25
  • ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1)
  • ras-related C3 botulinum toxin substrate 1
  • rho family, small GTP binding protein Rac1
  • TC25
  • TC-25

Background

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]. Transcript Variant: This variant (Rac1c) differs in the 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant Rac1. These differences cause translation initiation at a downstream ATG resulting in isoform Rac1c which is shorter compared to isoform Rac1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14907
Species: Mu, Rt
Applications: WB, IHC, IHC-P
NB100-91273
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-47602
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-01462
Species: Hu
Applications: WB, Flow, ICC/IF
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-52556
Species: Hu, Mu, Po, Bt, Bv, Ca, Mk, Pm, Rb
Applications: IHC-P
NBP1-33449
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-56159
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-21577
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF6260
Species: Hu
Applications: WB
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-45923
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85802
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Rac1 Lysate (NBL1-15104) (0)

There are no publications for Rac1 Lysate (NBL1-15104).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Rac1 Lysate (NBL1-15104) (0)

There are no reviews for Rac1 Lysate (NBL1-15104). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Rac1 Lysate (NBL1-15104) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Rac1 Products

Bioinformatics Tool for Rac1 Lysate (NBL1-15104)

Discover related pathways, diseases and genes to Rac1 Lysate (NBL1-15104). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rac1 Lysate (NBL1-15104)

Discover more about diseases related to Rac1 Lysate (NBL1-15104).
 

Pathways for Rac1 Lysate (NBL1-15104)

View related products by pathway.

PTMs for Rac1 Lysate (NBL1-15104)

Learn more about PTMs related to Rac1 Lysate (NBL1-15104).
 

Research Areas for Rac1 Lysate (NBL1-15104)

Find related products by research area.

Blogs on Rac1.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Rac1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RAC1
Entrez