Rabex5 Antibody

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Western Blot: Rabex5 Antibody [NBP1-55342] - HT1080 cell lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Product Discontinued
View other related Rabex5 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-55342
    • Availability
      Product Discontinued

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Rabex5 Antibody Summary

Immunogen
Synthetic peptides corresponding to RABGEF1(RAB guanine nucleotide exchange factor (GEF) 1) The peptide sequence was selected from the N terminal of RABGEF1. Peptide sequence MSLKSERRGIHVDQSDLLCKKGCGYYGNPAWQGFCSKCWREEYHKARQKQ.
Clonality
Polyclonal
Host
Rabbit
Gene
RABGEF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against RABGEF1 and was validated on Western blot.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Rabex5 Antibody

  • FLJ32302
  • RAB guanine nucleotide exchange factor (GEF) 1
  • rab5 GDP/GTP exchange factor
  • Rabaptin-5-associated exchange factor for Rab5
  • Rabex-5
  • RABEX5rabex-5
  • RAP1

Background

RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Rabex5 Antibody (NBP1-55342). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to know if NBP1-55342 is suitable for detecting their sequence: MAERLKHKSKIHERLNNPELRCKTGCGFYGNPAWQGYCSVCFREVYLKQH Would you please help compare and suggest?
    • NBP1-55342 is a polyclonal antibody. The peptide sequence was selected from the N terminal of RABGEF1 Peptide sequence MSLKSERRGIHVDQSDLLCKKGCGYYGNPAWQGFCSKCWREEYHKARQKQ. I did blast on the sequence you provided and that doesn't seem to be human RABGEF1, unfortunately I won't be able to assist your customer unless more information is provided. All I can say is that your customer could definitely use NBP1-55342 as longs as, the protein they are going to detect is human RABGEF1 and just in case they are using a truncated construct in transfected cells, the N-term should be intact. Please be advised that this antibody has only been validated in human.

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Bioinformatics

Gene Symbol RABGEF1
Uniprot