Rabbit IgG, Fab2 Native Protein

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SDS-Page: Rabbit IgG, Fab2 Native Protein [NBP1-97017] - SDS-PAGE of Rabbit IgG F(ab')2 Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab')2 ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IP, PAGE
Concentration
2.0 mg/ml

Order Details

Rabbit IgG, Fab2 Native Protein Summary

Description
Native Rabbit IgG, Fab2 protein.

Source: Rabbit Serum

Preparation
Method
Rabbit IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F(ab')2 and anti-Rabbit Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

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