Rabbit 20S Proteasome Protein, CF

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Rabbit 20S Proteasome Protein, CF Summary

Details of Functionality
The Rabbit 20S Proteasome is able to degrade substrates in an ATP-independent manner. It can be activated chemically with SDS (0.035%) or by the addition of PA28 (Cat # E-380 or E-381). Reaction conditions will need to be optimized for each specific application. We recommend an initial Rabbit 20S Proteasome concentration of 0.5-5 nM.
Source
Rabbit erythrocyte-derived 20S Proteasome protein
Protein/Peptide Type
Natural Enzymes
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
700 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E-350 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rabbit 20S Proteasome Protein, CF

  • 20S Proteasome

Background

The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins.  It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex (1). The 20S Proteasome is composed of 28 subunits arranged into four stacked rings (2,3). The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits.  The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core.  The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits.  The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention (4). The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator alpha (5). 

This product has been purified from rabbit erythrocytes.

  1. Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41:8.
  2. Kim, H.M. et al. (2011) Biochim. Biophys. Acta 1809:67.
  3. Xie, Y. (2010) J. Mol. Cell Biol. 2:308.
  4. Kisselev, A.F. et al. (2012) Chem. Biol. 19:99.
  5. Ma, C.P. et al. (1992) J. Biol. Chem. 267:10515.

Publications for 20S Proteasome (E-350)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioassay
(2)
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Human
(2)
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