Recombinant Human Rab27a Protein

Images

 
Western Blot: RAB27A Protein [NBP1-44380] - 15% SDS-PAGE (3ug)
SDS-Page: RAB27A Protein [NBP1-44380] - RAB27A, 27 kDa (241aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Rab27a Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-221 of Human Rab27a

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRASGPDG ATGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RVVKEEEAIA LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHASTDQL SEEKEKGACG C

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RAB27A
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Rab27a Protein

  • GS2
  • GS2rab-27
  • GTP-binding protein Ram
  • HsT18676
  • Rab-27
  • Rab27a
  • RAB27A, member RAS oncogene family
  • RAB27MGC117246
  • RAM
  • RAMras-related protein Rab-27A

Background

Ras-related protein Rab-27A, also known as RAB27A, belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Also, RAB27A regulates the cytotoxic granule exocytosis and affect T-lymphocyte and macrophage-activation. It is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Defects in RAB27A cause Griscelli syndrome type 2 (GS2). Recombinant human RAB27A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RAB27A
Entrez