PU.1/Spi-1 Antibody (EPR3158Y)

Images

 
Western Blot: PU.1/Spi-1 Antibody (EPR3158Y) [NBP1-40555] - (A) Daudi and (B) U937 cell lysates using a 1:10,000 dilution.
Immunohistochemistry: PU.1/Spi-1 Antibody (EPR3158Y) [NBP1-40555] - Analysis of paraffin-embedded human tonsils using anti-Transcription factor PU.1

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-40555
    • Availability
      Product Discontinued

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PU.1/Spi-1 Antibody (EPR3158Y) Summary

Immunogen
A synthetic peptide corresponding to residues near the N terminus of Transcription factor PU.1 was used as an immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SPI1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-10000
  • Flow Cytometry 1:70
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
Application Notes
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry. In Western blot this antibody detects a band at approximately 42kDa. This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)

Reactivity Notes

Cross reactivity determined by Western Blot only.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Purity
Protein A or G purified

Alternate Names for PU.1/Spi-1 Antibody (EPR3158Y)

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Additional PU.1/Spi-1 Products

Bioinformatics Tool for PU.1/Spi-1 Antibody (NBP1-40555)

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Diseases for PU.1/Spi-1 Antibody (NBP1-40555)

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Bioinformatics

Gene Symbol SPI1
Entrez
Uniprot