PTPN6 Antibody

Images

 
Western Blot: PTPN6 Antibody [NBP1-32179] - A. 50 ug rat liver extract 7.5% SDS-PAGE SHP1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PTPN6 Antibody [NBP1-32179] - Paraffin-embedded OVCA xenograft, using antibody at 1:100 dilution.
Western Blot: PTPN6 Antibody [NBP1-32179] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: PTPN6 Antibody [NBP1-32179] - Sample(30 ug of whole cell lysate)A:HeLaS3B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.
Immunoprecipitation: PTPN6 Antibody [NBP1-32179] - HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.52 mg/ml

Order Details

PTPN6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SHP1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.52 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PTPN6 Antibody

  • HCPH
  • HCPSH-PTP1
  • hematopoietic cell phosphatase
  • Hematopoietic cell protein-tyrosine phosphatase
  • HPTP1C
  • protein tyrosine phosphatase, non-receptor type 6
  • Protein-tyrosine phosphatase 1C
  • Protein-tyrosine phosphatase SHP-1
  • PTP1C
  • PTP-1CEC 3.1.3.48
  • SHP1
  • SHP-1
  • SHP-1L
  • tyrosine-protein phosphatase non-receptor type 6

Background

SHP-1 (also called PTP1C, SH-PP1, HCP, SHP and PTPN6) is a protein tyrosine phosphatase (PTP) predominantly expressed in hematopoietic cells, and regulates multiple signaling pathways involved in hematopoietic cell growth, differentiation and activation (1). After cell stimulation, SHP-1 (as well as SHP-2), translocate from cytosol to plasma membrane and bind to the tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). SHP-1 has been involved in the negative regulation of various growth promoting receptors including receptor tyrosine kinase (c-Kit, CSF-1, EGF), cytokine receptors (IL-3, IFN-a/b) and other tyrosine based receptor CD22, B & T cell Ag receptor, Lck, Zap-70 and Vav (3-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTPN6