PTIP Antibody

Images

 
Western Blot: PTIP Antibody [NB100-576] - Detection of Human PTIP on HeLa whole cell lysate using NB100-576. PTIP was also IPed using rabbit anti-PTIP antibody NB100-577 Immunoprecipitated PTIP was detected using BL1787.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-576
    • Availability
      Product Discontinued

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PTIP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 800 and 850 of human PAX transcription activation domain interacting protein 1 using the numbering given in TrEMBL entry Q6ZW49 (GeneID 22976).
Clonality
Polyclonal
Host
Rabbit
Gene
PAXIP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:25000
  • Immunoprecipitation 5-10 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:2,500 - 1:25,000 Immunoprecipitation: 5 to 10 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
PTIP Lysate (NBL1-14134)
Publications
Read Publications using NB100-576.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PTIP Antibody

  • CAGF28
  • PACIP1
  • PAX interacting (with transcription-activation domain) protein 1
  • PAX transactivation activation domain-interacting protein
  • PAX transcription activation domain interacting protein 1 like
  • PAX-interacting protein 1
  • PAXIP1LCAGF29
  • protein encoded by CAG trinucleotide repeats
  • PTIPFLJ41049
  • TNRC2

Background

PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Tandem BRCT (BRCA1 carboxyl-terminal) domains in PTIP and in BRCA1 serve as phosphoserine or phosphothreonine specific binding modules that recognize substrates phosphorylated by the kinases ATM and ATR in response to gamma irradiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTIP Antibody (NB100-576)(2)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for PTIP Antibody (NB100-576)

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Bioinformatics

Gene Symbol PAXIP1
Entrez
Uniprot