PSMC3 Antibody (9F3)

Images

 
Western Blot: PSMC3 Antibody (9F3) [NBP2-02316] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
Immunohistochemistry-Paraffin: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of paraffin-embedded prostate tissue using anti-PSMC3 mouse monoclonal antibody.
Flow Cytometry: PSMC3 Antibody (9F3) [NBP2-02316] - Analysis of Jurkat cells, using anti-PSMC3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: PSMC3 Antibody (9F3) [NBP2-02316] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMC3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of paraffin-embedded bladder tissue using anti-PSMC3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-PSMC3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMC3 Antibody (9F3) [NBP2-02316] - Staining of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
9F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PSMC3 Antibody (9F3) Summary

Immunogen
Full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSMC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSMC3 Antibody (9F3)

  • human immunodeficiency virus tat transactivator binding protein-1
  • MGC8487
  • proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5
  • Proteasome 26S subunit ATPase 3
  • Proteasome subunit P50
  • TBP-126S protease regulatory subunit 6A
  • TBP1Tat-binding protein 1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PSMC3
Entrez