PSGL-1/CD162 Antibody (KPL-1)

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Flow Cytometry: PSGL-1/CD162 Antibody (KPL-1) [NB100-78111] - Human peripheral blood lymphocytes stained with purified KPL-1, followed by anti-mouse IgG FITC

Product Details

Summary
Product Discontinued
View other related PSGL-1/CD162 Primary Antibodies

Order Details


    • Catalog Number
      NB100-78111
    • Availability
      Product Discontinued

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PSGL-1/CD162 Antibody (KPL-1) Summary

Immunogen
PSGL-1 transfected murine 300.19 pre B-cell line
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SELPLG
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry < = 0.5 ug/10^6 cells in 100 ul
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
WB, IP and IHC (paraffin and frozen sections) reported in literature.

Reactivity Notes

Cross-reactive with Cynomolgus monkey.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for PSGL-1/CD162 Antibody (KPL-1)

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional PSGL-1/CD162 Products

Bioinformatics Tool for PSGL-1/CD162 Antibody (NB100-78111)

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Diseases for PSGL-1/CD162 Antibody (NB100-78111)

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Bioinformatics

Gene Symbol SELPLG
Entrez
Uniprot