PSD-95 Antibody

Images

 
Western Blot: PSD95 Antibody [NBP1-51937] (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51937
    • Availability
      Product Discontinued

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PSD-95 Antibody Summary

Immunogen
internal region (near C-Terminus)
Epitope
C-EEQARKAFDRATK
Marker
post-Synaptic Marker
Specificity
This antibody is expected to recognize both reported isoforms (NP_001356.1; NP_001122299.1)
Clonality
Polyclonal
Host
Goat
Gene
DLG4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Peptide ELISA detection limit 1:8000
Application Notes
This antibody works in ELISA and Western Blot. Approx 110kDa band observed in Mouse Brain lysates (calculated MW of 85.4kDa according to Human NP_001356.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Chaudhury et al,, Am J Physiol Gastrointest Liver Physiol. 2009 Aug 13. PMID: 19679819). Recommended concentration: 1-3ug/ml.
Publications
Read Publication using NBP1-51937.

Reactivity Notes

Cross reacts with Mouse. Expected from sequence similarity: Human, Rat, Dog and Cow.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSD-95 Antibody

  • discs, large homolog 4 (Drosophila)
  • disks large homolog 4
  • DLG4
  • FLJ97752
  • FLJ98574
  • Postsynaptic density protein 95
  • post-synaptic density protein 95
  • PSD95
  • PSD-95
  • PSD95SAP90SAP-90discs large homolog 4
  • SAP90
  • Synapse-associated protein 90
  • Tax interaction protein 15

Background

Postsynaptic density protein 95 kDa (PSD-95), also known as Synapse associated protein 90 kDa (SAP90), is a brain specific protein that is highly similar to the Drosophila dlg tumor suppressor protein. PSD-95 is a member of membrane associated proteins that are localized beneath the postsynaptic membrane of synapses in the CNS. PSD-95 interacts with NMDA receptor and Shaker-type K+ channels and contributes to their clustering and localization at the synaptic spines in hippocampal neurons and the pinceau terminal of cerebellar basket cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSD-95 Antibody (NBP1-51937)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DLG4
Entrez
Uniprot