PSD-95 Antibody (S28-43)

Images

 
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (S28-43) [NBP1-47642] - Cultured mouse embryonic spinal neuron. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (S28-43) [NBP1-47642] - IF of PSD-95 in cultures of dissociated hippocampal neurons. Cultures were grown and labeled with antibodies against the indicated ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47642
    • Availability
      Product Discontinued

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PSD-95 Antibody (S28-43) Summary

Immunogen
Fusion protein amino acids 77-200 of human (NP_001122299.1).
Epitope
PSD95/SAP90
Marker
post-Synaptic Marker
Specificity
The product detects ~95-110kDa (varies with cell background due to phosphorylation).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DLG4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47642 in the following applications:

Publications
Read Publications using
NBP1-47642 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Monkey reactivity reported in scientific literature (PMID: 27155150).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PSD-95 Antibody (S28-43)

  • discs, large homolog 4 (Drosophila)
  • disks large homolog 4
  • DLG4
  • FLJ97752
  • FLJ98574
  • Postsynaptic density protein 95
  • post-synaptic density protein 95
  • PSD95
  • PSD-95
  • PSD95SAP90SAP-90discs large homolog 4
  • SAP90
  • Synapse-associated protein 90
  • Tax interaction protein 15

Background

Postsynaptic density protein 95 kDa (PSD-95), also known as Synapse associated protein 90 kDa (SAP90), is a brain specific protein that is highly similar to the Drosophila dlg tumor suppressor protein. PSD-95 is a member of membrane associated proteins that are localized beneath the postsynaptic membrane of synapses in the CNS. PSD-95 interacts with NMDA receptor and Shaker-type K+ channels and contributes to their clustering and localization at the synaptic spines in hippocampal neurons and the pinceau terminal of cerebellar basket cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSD-95 Antibody (NBP1-47642)(4)

We have publications tested in 2 confirmed species: Rat, Monkey.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(3)
All Applications
Filter By Species
Rat
(2)
Monkey
(1)
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Showing Publications 1 - 4 of 4.
Publications using NBP1-47642 Applications Species
Miller S, Liu H, Warfvinge K et al. Immunohistochemical localization of the calcitonin gene-related peptide binding site in the primate trigeminovascular system using functional antagonist antibodies. Neuroscience. Jul 22 2016 [PMID: 27155150] (IHC-P, Monkey) IHC-P Monkey
Altabella L, Sbriccoli M, Zoratto F et al. Differential responses to acute administration of a new 5-HT7-R agonist as a function of adolescent pre-treatment: phMRI and immuno-histochemical study. Front Behav Neurosci. 2015 Jan 08 [PMID: 25565998] (IHC-P, Rat) IHC-P Rat
Cline H. Synaptogenesis: a balancing act between excitation and inhibition. Curr Biol;15(6):R203-5. 2005 Mar 29. [PMID: 15797012]
Altabella L, Sbriccoli M, Zoratto F. Differential responses to acute administration of a new 5-HT7-R agonist asa function of adolescent pre-treatment: phMRI and immuno-histochemicalstudy name: Frontiers in behavioral neuroscience et al. 2014 (IHC-P, Rat)

Details:
PSD-95 antibody used at 1:500 dilution for IHC-P on sections of brain hemispheres from Rats subjected to acute administration of LP-211 [selective agonist of serotonin (5-hydroxytryptamine, 5-HT) receptor 7 (5-HT7-R)], or vehicle.
IHC-P Rat

Review for PSD-95 Antibody (NBP1-47642) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-47642:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry PSD-95 NBP1-47642
Enlarge
reviewed by:
Jianning Lu
ICC Mouse 09/02/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured mouse embryonic spinal neuron
SpeciesMouse
Lot1007

Blocking

Blocking Details10% horse serum with 0.5% Triton X100 in PBS, 1 hour @ RT

Primary Anitbody

Dilution Ratio1:300, 1% horse serum with 0.1% Triton X100 in PBS, overnight @ 4 °C

Secondary Antibody

Secondary DescriptionDonkey Anti-Mouse IgG (H+L) Alexa Fluor® 594 from Invitrogen
Secondary Manufacturer Cat#A-21203
Secondary Concentration1:700

Details

Detection NotesConfocal microscopy with Leica SP2 microscope
Fixation DetailsFixed with 4% PFA, 10 min @ RT; permeablized with 0.5% Triton X100 in PBS, 15 min @ RT
Wash DescriptionPBS, 15 min for 3 times

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSD-95 Antibody (NBP1-47642) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Jianning Lu
09/02/2014
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol DLG4
Entrez
Uniprot