Prothrombin RNAi

Images

 
Western Blot: Prothrombin RNAi [H00002147-R01V] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi or non-transfected control. Blot probed with H00002147-M01. GAPDH (36.1 kDa) ...read more
RNAi sequence position: Prothrombin RNAi [H00002147-R01V]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, RNAi, RNAi SP

Order Details

Prothrombin RNAi Summary

Specificity
Homo sapiens coagulation factor II (thrombin), mRNA
Gene
F2

Applications/Dilutions

Application Notes
This RNAi causes effective protein knockdown which has been antibody validated.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Prothrombin RNAi

  • coagulation factor II (thrombin)
  • Coagulation factor II
  • EC 3.4.21
  • EC 3.4.21.5
  • prothrombin B-chain
  • prothrombin
  • PT
  • serine protease

Background

Position of the Chimera RNAi. The related RNAi products listed were designed from different accesion number but sharing the same RNAi sequence. Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene.  The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.  Chimera RNAi has many advantages over the conventional siRNAs.  First, it has been demonstrated to have reliable knock-down for over 10,000 human genes.  Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence.  Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected.  Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Bioinformatics Tool for Prothrombin RNAi (H00002147-R01V)

Discover related pathways, diseases and genes to Prothrombin RNAi (H00002147-R01V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Prothrombin RNAi (H00002147-R01V)

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Blogs on Prothrombin.

Factor VIII - a key factor in the clotting process
Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation...  Read full blog post.

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Bioinformatics

Gene Symbol F2