Prothrombin Antibody (1B7)

Images

 
Western Blot: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected ...read more
Western Blot: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Proximity Ligation Assay: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 ...read more
Immunoprecipitation: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal ...read more
Sandwich ELISA: Prothrombin Antibody (1B7) [H00002147-M01] - Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP, PLA, RNAi
Clone
1B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Prothrombin Antibody (1B7) Summary

Immunogen
F2 (AAH51332 263 a.a. - 362 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG
Localization
Secreted
Specificity
F2 - coagulation factor II (thrombin)
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
F2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
  • RNA Inhibition
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Prothrombin Antibody (1B7)

  • coagulation factor II (thrombin)
  • Coagulation factor II
  • EC 3.4.21
  • EC 3.4.21.5
  • prothrombin B-chain
  • prothrombin
  • PT
  • serine protease

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Factor VIII - a key factor in the clotting process
Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation...  Read full blog post.

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Bioinformatics

Gene Symbol F2
Entrez
OMIM
Uniprot