Protein L Type 1 Recombinant Protein Summary
A recombinant protein corresponding to Peptostreptococcus Magnus Protein L Type 1
Source: E. coli
Amino Acid Sequence: The amino acid sequence of this product is not available.
|Details of Functionality
Binding Capacity:Under optimal conditions, 1 mg protein L will bind approximately 5 mg human IgG. Optimal binding of Protein L to antibodies occurs at pH 7.5 over a pH range of 2.5 to 3.0.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Reconstitute with water to a final concentration of 5.0 mg/ml.
After reconstituted, solution is clear.
Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. 's Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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