Protein Kinase D2 Antibody (5A3)

Images

 
Western Blot: Protein Kinase D2 Antibody (5A3) [NBP2-02968] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody (5A3) [NBP2-02968] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5A3) [NBP2-02968] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Protein Kinase D2 mouse monoclonal antibody.
Flow Cytometry: Protein Kinase D2 Antibody (5A3) [NBP2-02968] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein Kinase D2 ...read more
Flow Cytometry: Protein Kinase D2 Antibody (5A3) [NBP2-02968] - Analysis of Hela cells, using anti-Protein Kinase D2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Protein Kinase D2 Antibody (5A3) [NBP2-02968] - Analysis of Jurkat cells, using anti-Protein Kinase D2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02968
    • Availability
      Product Discontinued

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Protein Kinase D2 Antibody (5A3) Summary

Immunogen
Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRKD2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Protein Kinase D2 Antibody (5A3)

  • DKFZP586E0820
  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-D2
  • PKD2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2

Background

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRKD2
Entrez