Protein G Antibody

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Western Blot: Protein G Antibody [NBP1-50763] - 10 ng recombinant protein G.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, GP, Gt, Rb, ShSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Protein G Antibody Summary

Immunogen
Recombinant Protein G
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant Protein G can be used as a positive control.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Protein G Antibody

  • IgG binding protein G Immunoglobulin G binding protein G [Precursor] spg

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Protein G Antibody (NBP1-50763). (Showing 1 - 1 of 1 FAQ).

  1. I have a question regarding a polyclonal product, NBP1-50763, polyclonal specific for recombinant protein G. 1. Is the recombinant used as immunogen one that has had the albumin binding domain removed? 2. Affinity purification -- general purification of IG, or specifically purified anti-protein G, and if so, how?
    • For the recombinant protein G, the albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure maximum specific IgG binding capacity. The antibody is then purified using protein G-beads.

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