Proteasome 20S C2 Antibody

Images

 
Western Blot: Proteasome 20S C2 Antibody [NB600-1016] - Luminograph of human erythrocyte-derived 20S proteasome lysate after SDS-PAGE followed by blotting onto PVDF and probing with antibody NB600-1016. Antibody ...read more

Product Details

Summary
Product Discontinued
View other related Proteasome 20S C2 Primary Antibodies

Order Details


    • Catalog Number
      NB600-1016
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Proteasome 20S C2 Antibody Summary

Immunogen
A proteasomal preparation isolated from human red blood cells.
Localization
Cytoplasmic and Nuclear.
Specificity
This detects a combination of alpha and beta proteasome subunits.
Clonality
Polyclonal
Host
Rabbit
Gene
PSMA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Western blotting - The antibody has been characterized by single dimension SDS-PAGE using both purified human and yeast 20S proteasome and a number of tissue/cell preparations including a human placental proteasome preparation, a HeLa cell lysate, a RSV 3T3 mouse fibroblast cell lysate, and a yeast whole cell extract.
Publications
Read Publications using
NB600-1016 in the following applications:

  • WB
    1 publication

Reactivity Notes

Cross-reacts with S. cerevisiae proteasomes.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS
Preservative
10mM Sodium Azide
Purity
Unpurified

Alternate Names for Proteasome 20S C2 Antibody

  • EC 3.4.25.1
  • HC2Proteasome nu chain
  • Macropain subunit C2
  • macropain subunit nu
  • MGC14542
  • MGC14575
  • MGC14751
  • MGC1667
  • MGC21459
  • MGC22853
  • MGC23915
  • Multicatalytic endopeptidase complex subunit C2
  • NU30 kDa prosomal protein
  • PROS-30
  • PROS30Proteasome component C2
  • proteasome (prosome, macropain) subunit, alpha type, 1
  • proteasome subunit alpha type-1
  • proteasome subunit nu
  • proteasome subunit, alpha-type, 1
  • protein P30-33K
  • PSC2

Background

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450A in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha-subunits comprise the outer rings and the beta-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha 7 beta 7 beta 7 alpha 7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the alpha- or beta-rings (ref 1). 20S Proteasomes degrade only unfolded proteins in an energy-independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP-dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome (ref 2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB6240
Species: Hu
Applications: WB, IHC
NBP1-36999
Species: Hu, Mu, Rt
Applications: PEP-ELISA
NBP2-24614
Species: Hu, Mu, Rt, Op, Pm
Applications: WB
NBP2-24763
Species: Hu, Pm
Applications: WB, IHC-P
NB100-55268
Species: Hu
Applications: WB, IP
NBP1-87343
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NBP1-77933
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-38621
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
MAB29051
Species: Hu
Applications: IP, CyTOF-ready, ICC, ICFlow
AF4779
Species: Hu
Applications: WB, IHC
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF6144
Species: Hu
Applications: WB, Simple Western
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-05027
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, PEP-ELISA, S-ELISA

Publications for Proteasome 20S C2 Antibody (NB600-1016)(7)

Reviews for Proteasome 20S C2 Antibody (NB600-1016) (0)

There are no reviews for Proteasome 20S C2 Antibody (NB600-1016). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Proteasome 20S C2 Antibody (NB600-1016) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Proteasome 20S C2 Products

Bioinformatics Tool for Proteasome 20S C2 Antibody (NB600-1016)

Discover related pathways, diseases and genes to Proteasome 20S C2 Antibody (NB600-1016). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Proteasome 20S C2 Antibody (NB600-1016)

Discover more about diseases related to Proteasome 20S C2 Antibody (NB600-1016).
 

Pathways for Proteasome 20S C2 Antibody (NB600-1016)

View related products by pathway.

PTMs for Proteasome 20S C2 Antibody (NB600-1016)

Learn more about PTMs related to Proteasome 20S C2 Antibody (NB600-1016).
 

Research Areas for Proteasome 20S C2 Antibody (NB600-1016)

Find related products by research area.

Blogs on Proteasome 20S C2

There are no specific blogs for Proteasome 20S C2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Proteasome 20S C2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PSMA1
Entrez
Uniprot