Recombinant Human Proteasome 20S beta 7 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human Proteasome 20S beta 7 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 44-277 of Human Proteasome 20S beta 7

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTA ADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPY TVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PSMB7
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human Proteasome 20S beta 7 Protein

  • EC 3.4.25.1
  • Macropain chain Z
  • Multicatalytic endopeptidase complex chain Z
  • proteasome (prosome, macropain) subunit, beta type, 7
  • proteasome subunit alpha
  • proteasome subunit beta 7
  • proteasome subunit beta type-7
  • Proteasome subunit Z
  • PSMB7
  • Zproteasome catalytic subunit 2

Background

PSMB7, also known as proteasome subunit beta type-7, is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i. Recombinant human PSMB7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PSMB7