Proteasome 20S beta 7 Antibody

Images

 
Western Blot: Proteasome 20S beta 7 Antibody [NBP1-52081] - NBP1-52081 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Proteasome 20S beta 7 Antibody Summary

Immunogen
Peptide with sequence C-RNAVLEADFAKR corresponding to internal region according to NP_002790.1.
Epitope
C-RNAVLEADFAKR
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSMB7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 40 kDa band observed in lysates of HEK 293, HeLa and Jurkat and of mouse Fetal brain (calculated MW of 30.0 kDa band according to NP_002790.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-52081.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Proteasome 20S beta 7 Antibody

  • EC 3.4.25.1
  • Macropain chain Z
  • Multicatalytic endopeptidase complex chain Z
  • proteasome (prosome, macropain) subunit, beta type, 7
  • proteasome subunit alpha
  • proteasome subunit beta 7
  • proteasome subunit beta type-7
  • Proteasome subunit Z
  • PSMB7
  • Zproteasome catalytic subunit 2

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Proteasome 20S beta 7 Antibody (NBP1-52081)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMB7
Entrez
Uniprot