Prostatic Acid Phosphatase/ACPP Antibody

Product Discontinued

Prostatic Acid Phosphatase/ACPP Antibody Summary

• Description: Purification Notes
  After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared ( anti-PAP IgY fraction ). Some of this antibody was further purified using an agarose matrix to which the PAP protein was covalently attached ( Affinity-purified anti-PAP ). The final preparation in the accompanying vial contains 10 mg/ml of the anti-PAP IgY fraction supplemented with 20 mg/ml of the affinity-purified anti-PAP plus 50% (v/v) glycerol (to prevent freezing at 20 C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 ul aliquots prepared.
  
  Storage Notes
  Store at -20C in the dark. The antibody should have a shelf life of at least 2 years (provided it is kept under sterile conditions). For longer storage periods, store at -80C. Note that storage at this lower temperature will destroy some antibody activity due to water crystallization.
• Immunogen: Chickens were immunized with recombinant mouse Prostatic Acid Phosphatase protein.
• Marker: Neuron Cell Marker
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: ACP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1:1000-1:2000
• Application Notes: Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAP-positive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, were used at a concentration of 1:500.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 10 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Prostatic Acid Phosphatase/ACPP Antibody

• acid phosphatase, prostate
• ACP3
• ACP-3
• ACPP
• EC 3.1.3.2
• PAP
• Prostatic Acid Phosphatase
• prostatic acid phosphotase

Background

Prostatic Acid Phosphatase encodes an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and secreted by the epithelial cells of the prostrate gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACP3