Prominin 2 Antibody (14D1)

Images

 
Western Blot: Prominin 2 Antibody (14D1) [NBP1-47941] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prominin 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Prominin 2 Antibody (14D1) [NBP1-47941] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prominin 2.
Immunohistochemistry-Paraffin: Prominin 2 Antibody (14D1) [NBP1-47941] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-Prominin 2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Prominin 2 Antibody (14D1) [NBP1-47941] - Immunofluorescent staining of Cos7 cells using anti-Prominin 2 mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47941
    • Availability
      Product Discontinued

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Prominin 2 Antibody (14D1) Summary

Immunogen
Protein expressed in 293T cell transfected with human PROM2 expression vector
Specificity
This antibody is specific for Homo sapiens prominin 2 (PROM2), transcript variant 3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PROM2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Prominin 2 Antibody (14D1)

  • hPROML2
  • MGC138714
  • PROM2
  • PROM-2
  • Prominin 2
  • prominin-2
  • Prominin-like protein 2
  • prominin-related protein
  • PROML2

Background

A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. Several criteria were applied to identify and characterize these new and novel genes. First, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins, second strategy utilized various algorithms that recognize features such as the hydrophobic properties of signal sequences to identify putative proteins encoded by expressed sequence tags (ESTs) from human cDNA libraries and finally, ESTs for protein sequence similarity to a set of known receptors and their ligands with the BLAST algorithm. Based on these criteria isolation of full length cDNA clones for each of these genes resulted in identification of more than 1000 novel proteins. One of these new genes is Prominin2. Prominin 2 is a 112 kDa glycoporotein structurally related to Prominin 1 (CD133) although amino acid similarity is not more than 30% but their genomic organization is strikingly similar (1). Like Prominin1, the prominin 2 exhibit similar membrane topology with 5 trans-membrane domains and two large glycosylated extracellular domains. Similar to Prominin1 localization, the Prominin 2 is also associated with membrane protrusions of the epithelial cells from adult kidney, and all along the digestive track and other epithelial tissues. One striking difference between Prominin1 and Prominin 2 expression lies in its conspicuous absence in eye tissue (2). Prominin 2, along with other genes (AP1M2, MAL2, PRSS8 and FLJ20171) expression is differentially expressed in chromophobe renal cell carcinoma compared to benign oncocytoma consistently and this marker can be used to differentiate RCC form benign oncocytoma (3). Prominin 2 is an androgen-responsive genes proapoptotic membrane protein that is expressed in human prostate and human prostate cancer cell lines with highest levels in less aggressive LNCaP cell and low expression in highly aggressive PC3 and DU145 cells suggesting a role of Prominin 2 in down-regulation of aggressiveness of prostate cancer cells (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PROM2
Entrez
Uniprot