Prohibitin 2 Antibody

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Western Blot: Prohibitin 2 Antibody [NB120-18305] - MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Prohibitin 2 Antibody Summary

Immunogen
Partial recombinant BAP37 protein at the C-terminus.
Localization
Mitochondrial
Specificity
Reacts with the estrogen receptor, prohibitin, and IgM.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 10ul/5ml
Application Notes
It is recommended that the reagent be titrated for optimal performance for each application. The WB dilution refers to a mini-gel.
Positive Control
MCF-7 Lysate (NB820-59465)

Reactivity Notes

Cross-reacts with Human and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Prohibitin 2 Antibody

  • BAP
  • Bap37
  • BCAP37
  • B-cell associated protein
  • D-Prohibitin
  • MGC117268
  • p22
  • PHB2
  • PNAS-141
  • Prohibitin 2
  • prohibitin-2
  • REA
  • REAB-cell receptor-associated protein BAP37
  • Repressor of estrogen receptor activity

Background

BAP37, (also known as B-cell receptor-associated protein, repressor of estrogen receptor activity, and prohibitin 2) is a 37 kD erythrocyte band 7 integral membrane protein that contains an SPFH domain. BAP37 is located in the inner mitochondrial membrane that selectively potentiates the inhibitory effectiveness of antiestrogen-occupied ER. BAP37 plays a possible role in cell aging and acts as a mitochondrial protein chaperone. This protein has been shown to interact with the estrogen receptor, prohibitin, and IgM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Prohibitin 2 Antibody (NB120-18305) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PHB2
Entrez