Progesterone R/NR3C3 Antibody

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Western Blot: Progesterone R/NR3C3 Antibody [NB100-91936] - Analysis of Progesterone R/NR3C3 (V184) antibody in extracts from COS7 treated with EGF.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91936
    • Availability
      Product Discontinued

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Progesterone R/NR3C3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 151-200 of Human PR.
Specificity
Progesterone Receptor (V184) detects endogenous levels of Progesterone Receptor protein.
Clonality
Polyclonal
Host
Rabbit
Gene
PGR
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Progesterone Receptor (V184) pAb in extracts from COS7 treated with EGF.
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Progesterone R/NR3C3 Antibody

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

The effects of progesterone are mediated by two functionally different isoforms of the progesterone receptor, PR-A and PR-B, which are transcribed from distinct, estrogen inducible promoters within a single copy of the PR gene. The PR-A and PR-B proteins are 94 kDa and 114 kDa respectively; the first 164 amino acids of PR-B are absent in PR-A. Progesterone bound PR-A and PR-B have different transcription activation properties. Specifically, PR-B functions as a transcriptional activator in most cell and promoter contexts, while PR-A is transcriptionally inactive and functions as a strong ligand dependent transdominant repressor of steroid hormone receptor transcriptional activity. An inhibitory domain (ID), which maps to the amino terminus of the receptor, exists within both PR isoforms. Interestingly, the ID is functionally active only in PR-A and is necessary for steroid hormone transrepression by PR-A, suggesting that PR-A and PR-B may have different conformations in the cell. Phosphorylation of human PR occurs on at least nine serine residues. Phosphorylation of three of the residues is hormone-inducible (Ser 102, Ser 294 and Ser 345); the others are basal but hormone-stimulated. The human PR gene maps to 11q13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Progesterone R/NR3C3 Antibody (NB100-91936). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Secondary Antibodies

 

Isotype Controls

Additional Progesterone R/NR3C3 Products

Bioinformatics Tool for Progesterone R/NR3C3 Antibody (NB100-91936)

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Diseases for Progesterone R/NR3C3 Antibody (NB100-91936)

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Bioinformatics

Gene Symbol PGR
Entrez