proBNP Antibody (50E1)

Images

 
Western Blot: proBNP Antibody (50E1) [NB200-440] - Analysis using the Biotin conjugate of NB200-440. Detection of synthetic huBNP in WB after Tricine-SDS-PAGE in reducing conditions.
Western Blot: proBNP Antibody (50E1) [NB200-440] - Immunodetection of human synthetic BNP (A), recombinant proBNP (E. coli) (B) and recombinant proBNP (mammalian cell line) (C) in Western blotting by different ...read more
Immunoassay: proBNP Antibody (50E1) [NB200-440] - Gel-filtration studies of endogenous proBNP and BNP. BNP immunoreactivity measurements in fractions after proteins from plasma of HF patients were separated by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IA, S-ELISA, CyTOF-ready
Clone
50E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

proBNP Antibody (50E1) Summary

Immunogen
Synthetic peptide (32 amino acids corresponding to a.a. 77-108 of proBNP molecule), conjugated with carrier protein. (Human).
Localization
Secreted
Specificity
Detects Human BNP and proBNP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NPPB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunoassay
  • Sandwich ELISA 1:100-1:2000
  • CyTOF-ready
Application Notes
Recommended pairs for BNP sandwich immunoassay (capture-detection): NB200-440 - NB600-1183. Sensitivity of the pair 50E1 - 24C5 is better than 1 pg/ml (synthetic BNP, Bachem). Recognizes with high sensitivity the antigen in plasma samples of patients with heart failure. MAb 50E1 can be used for proBNP detection in pairs with anti-NTproBNP (NB200-439 or NB100-62134).
Theoretical MW
14.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
proBNP Lysate (NBL1-13753)

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for proBNP Antibody (50E1)

  • BNP
  • brain type natriuretic peptide
  • Gamma-brain natriuretic peptide
  • natriuretic peptide B
  • natriuretic peptide precursor B
  • natriuretic peptides B
  • natriuretic protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for proBNP Antibody (NB200-440) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB200-438

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Bioinformatics

Gene Symbol NPPB
Entrez
Uniprot