PRMT5 Antibody

Images

 
Western Blot: PRMT5 Antibody [H00010419-D01P] - Analysis of PRMT5 expression in transfected 293T cell line by PRMT5 polyclonal antibody.Lane 1: PRMT5 transfected lysate(72.70 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PRMT5 Antibody Summary

Immunogen
PRMT5 (NP_006100.2, 1 a.a. ~ 637 a.a) full-length human protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRMT5
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
This antibody is useful for Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRMT5 Antibody

  • EC 2.1.1.-
  • EC 2.1.1.125
  • Histone-arginine N-methyltransferase PRMT5
  • HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein
  • HRMT1L5
  • IBP72
  • Jak-binding protein 1
  • JBP1
  • protein arginine methyltransferase 5
  • protein arginine N-methyltransferase 5
  • Shk1 kinase-binding protein 1 homolog
  • skb1 (S. pombe) homolog
  • SKB1 homolog (S. pombe)
  • SKB1
  • SKB1HsSKB1 homolog

Background

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRMT5
Uniprot