PRMT2 Antibody

Images

 
Western Blot: PRMT2 Antibody [NBP1-52943] - 293T cells lysate, concentration 0.2-1 ug/ml.
Chromatin Immunoprecipitation: PRMT2 Antibody [NBP1-52943] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-52943
    • Availability
      Product Discontinued

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PRMT2 Antibody Summary

Immunogen
Synthetic peptides corresponding to PRMT2(protein arginine methyltransferase 2) The peptide sequence was selected from the N terminal of PRMT2. Peptide sequence ERAGCCGYIPANHVGKHVDEYDPEDTWQDEEYFGSYGTLKLHLEMLADQP.
Clonality
Polyclonal
Host
Rabbit
Gene
PRMT2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Chromatin Immunoprecipitation
Application Notes
This is a rabbit polyclonal antibody against PRMT2 and was validated on Western blot.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for PRMT2 Antibody

  • EC 2.1.1.-
  • EC 2.1.1.125
  • Histone-arginine N-methyltransferase PRMT2
  • HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1
  • HMT1 hnRNP methyltransferase-like 1 (S. cerevisiae)
  • HMT1 hnRNP methyltransferase-like 1
  • HMT1
  • HRMT1L1PRMT2 beta
  • MGC111373
  • PRMT2 alpha
  • PRMT2 gamma
  • protein arginine methyltransferase 2
  • protein arginine N-methyltransferase 2

Background

The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PRMT2
Uniprot