Recombinant Human PRMT1 Protein

Images

 
SDS-Page: PRMT1 Protein [NBC1-18446] - PRMT1 isoform3, 84 kDa (750 aa) with a purity of 90% by SDS - PAGE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human PRMT1 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-MBP tag and corresponding to the amino acids 1-353 of Human PRMT1

Source: E.coli

Amino Acid Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

Details of Functionality
10,000unit/ml. One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PRMT1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
40mM Tris pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT, 40% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PRMT1 Protein

  • ANM1
  • EC 2.1.1
  • EC 2.1.1.-
  • EC 2.1.1.125
  • EC 2.1.1.77
  • HCP1
  • Histone-arginine N-methyltransferase PRMT1
  • HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
  • HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
  • HMT1
  • HMT2
  • HRMT1L2
  • HRMT1L2HMT1 hnRNP methyltransferase-like 2
  • Interferon receptor 1-bound protein 4
  • IR1B4
  • IR1B4protein arginine N-methyltransferase 1
  • PRMT1
  • protein arginine methyltransferase 1

Background

Protein arginine N-methyltransferase 1(PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-342
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-06603
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC
NBP2-01291
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-07996
Species: Hu
NBP2-52486
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
NBL1-11569
Species: Hu
H00008360-R01
Species: Hu
NBL1-11570
Species: Hu
NBP2-10888
Species: Hu
H00008365-M01
Species: Hu
Applications: ELISA, ICC/IF
NBL1-11567
Species: Hu
NB21-2024
Species: Hu, Mu, Rt, Ma
Applications: WB, ChIP, DB, ICC/IF
NB100-56641
Species: Hu, Mu
Applications: WB
NBP1-83205
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-86092
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19151
Species: Hu, Ma
Applications: WB, IHC-P, IP
NB100-381
Species: Hu
Applications: WB, ChIP, IP, PLA
NB100-56642
Species: Hu, Mu
Applications: WB

Publications for PRMT1 Biologically Active Protein (NBC1-18446) (0)

There are no publications for PRMT1 Biologically Active Protein (NBC1-18446).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PRMT1 Biologically Active Protein (NBC1-18446) (0)

There are no reviews for PRMT1 Biologically Active Protein (NBC1-18446). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for PRMT1 Biologically Active Protein (NBC1-18446) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PRMT1 Products

Bioinformatics Tool for PRMT1 Biologically Active Protein (NBC1-18446)

Discover related pathways, diseases and genes to PRMT1 Biologically Active Protein (NBC1-18446). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PRMT1 Biologically Active Protein (NBC1-18446)

Discover more about diseases related to PRMT1 Biologically Active Protein (NBC1-18446).
 

Pathways for PRMT1 Biologically Active Protein (NBC1-18446)

View related products by pathway.

PTMs for PRMT1 Biologically Active Protein (NBC1-18446)

Learn more about PTMs related to PRMT1 Biologically Active Protein (NBC1-18446).
 

Research Areas for PRMT1 Biologically Active Protein (NBC1-18446)

Find related products by research area.

Blogs on PRMT1.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

Customers Who Bought This Also Bought

PRMT6 Antibody
NB100-56642
PRMT1 Antibody
NB100-2251

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human PRMT1 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol PRMT1
Entrez