PRC1 Antibody (EP1513Y)

Product Discontinued

PRC1 Antibody (EP1513Y) Summary

• Immunogen: A synthetic peptide corresponding to residues on human PRC1 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:100-250
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:10
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 72 kDa.
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PRC1 Overexpression Lysate

Reactivity Notes

Human, Mouse, Rat. Cross reactivity determined by Western Blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for PRC1 Antibody (EP1513Y)

• anaphase spindle elongation 1 homolog
• ASE1
• MGC1671
• MGC3669
• protein regulating cytokinesis 1
• protein regulator of cytokinesis 1

Background

Novel human protein Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis (1). PRC1, a mitotic spindle-associated Cdk substrate that is essential to cell cleavage, is a microtubule binding and bundling protein both in vivo and in vitro. Over expression of PRC1 extensively bundles interphase microtubules, but does not affect early mitotic spindle organization (2). PRC1 has been shown to down-regulate the GAP activity of MgcRac-GAP during the metaphase and thereby contributes to the correct formation of the spindle (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRC1
• Entrez:
  • 9055(Human)
  • 233406(Mouse)
  • 308761(Rat)
• Uniprot:
  • Q9BSB6(Human)