PRAME Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

Order Details

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Catalog# & Conjugate Size Price

PRAME Antibody [DyLight 405] Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human PRAME (within residues 1-100). [Swiss-Prot# P78395]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRAME
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This PRAME antibody is useful for Western blot, where a band is seen at ~57 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PRAME Antibody [DyLight 405]

  • cancer/testis antigen 130
  • MAPEOIP4CT130
  • melanoma antigen preferentially expressed in tumors
  • OIP-4
  • Opa-interacting protein 4
  • Opa-interacting protein OIP4
  • preferentially expressed antigen in melanoma
  • Preferentially expressed antigen of melanoma

Background

PRAME (melanoma antigen preferentially expressed in tumors; OIP-4 or MAPE) is a transcriptional repressor factor which acts as a negative regulator of retinoic acid signaling through its interactions with retinoic acid receptors (RARA, RARB and RARG) and inhibits retinoic acid-mediated cell cycle arrest, cellular differentiation and apoptotic cell death. PRAME directlty interacts with RARA via its ligand-binding domain in ligand-dependent manner, and it also interacts with EZH2 which is required for RAR signaling repression. Localized in nucleus and cell membranes, PRAME is expressed in multiple tissues that include testis, kidney, brain, skin, ovary, adrenal, placenta and endometrium. PRAME was initially discovered as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumour-specific cytotoxic T lymphocytes derived from a melanoma patient. PRAME's expression profile defines it as a cancer-testis antigen (CTA). CTAs are encoded by non-mutated genes expressed at high levels in germinal tissues/tumours, but are absent from or detected at low levels in other tissues (MAGE, BAGE, GAGE and MAPE/PRAME protein families), however, PRAME may be somewhat different to other CTAs in that it shows expression in normal tissues also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRAME