PRAK Antibody

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Western Blot: PRAK Antibody [NBP1-41083] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-MK5 antibody used for WB at 0.04 ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-41083
    • Availability
      Product Discontinued

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PRAK Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 423 and 473 of human mitogen-activated protein kinase-activated protein kinase 5 using the numbering given in entry NP_620777.1 (GeneID 8550).
Clonality
Polyclonal
Host
Rabbit
Gene
MAPKAPK5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRAK Antibody

  • EC 2.7.11
  • MAP kinase-activated protein kinase 5
  • MAPK-activated protein kinase 5
  • MAPKAP kinase 5
  • MAPKAPK-5
  • mitogen-activated protein kinase-activated protein kinase 5
  • p38-regulated/activated protein kinase
  • PRAKEC 2.7.11.1

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPKAPK5
Entrez