PPP3CB Antibody

Images

 
Western Blot: PPP3CB Antibody [NBP1-32567] - Sample(30 ug whole cell lysate)A:A431 B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PPP3CB Antibody [NBP1-32567] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PPP3CB protein stained by PPP3CB antibody. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PPP3CB Antibody [NBP1-32567] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.
Western Blot: PPP3CB Antibody [NBP1-32567] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Western Blot: PPP3CB Antibody [NBP1-32567] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.49 mg/ml

Order Details

PPP3CB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PPP3CB. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP3CB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.49 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPP3CB Antibody

  • Calmodulin-dependent calcineurin A subunit beta isoform
  • CALNA2protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform(calcineurin A beta)
  • CAM-PRP catalytic subunit
  • CNA2catalytic subunit, beta isoform
  • EC 3.1.3.16
  • protein phosphatase 2B, catalytic subunit, beta isoform
  • protein phosphatase 3, catalytic subunit, beta isozyme
  • protein phosphatase from PCR fragment H32
  • serine/threonine-protein phosphatase 2B catalytic subunit beta isoform

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPP3CB
Entrez
Uniprot