PPAR gamma/NR1C3 Antibody

Images

 
Western Blot: PPAR gamma/NR1C3 Antibody [NBP1-77833] - Detection of PPAR protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PPAR gamma/NR1C3 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
Specificity
This affinity purified antibody is directed against human PPAR gamma isoform 2 protein. A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from mouse. Mouse PPARg2 shows 87% homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:4000-1:40000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a single band approximately 55 kDa in size corresponding - PPARg2 by western blot in the appropriate tissue or cell lysate.

Reactivity Notes

Reactive in human, macaque

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody

  • CIMT1
  • NR1C3
  • NR1C3GLM1
  • Nuclear receptor subfamily 1 group C member 3
  • peroxisome proliferator-activated receptor gamma 1
  • peroxisome proliferator-activated receptor gamma
  • PPAR gamma
  • PPARG
  • PPARG1peroxisome proliferative activated receptor, gamma
  • PPARG2PPARgamma
  • PPAR-gamma

Background

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs,

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
AF1443
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-04676
Species: Hu, Mu, Rt, Ha, Sq
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-77833
Species: Hu, Pm
Applications: WB, ELISA

Publications for PPAR gamma/NR1C3 Antibody (NBP1-77833) (0)

There are no publications for PPAR gamma/NR1C3 Antibody (NBP1-77833).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PPAR gamma/NR1C3 Antibody (NBP1-77833) (0)

There are no reviews for PPAR gamma/NR1C3 Antibody (NBP1-77833). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PPAR gamma/NR1C3 Antibody (NBP1-77833) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PPAR gamma/NR1C3 Products

Bioinformatics Tool for PPAR gamma/NR1C3 Antibody (NBP1-77833)

Discover related pathways, diseases and genes to PPAR gamma/NR1C3 Antibody (NBP1-77833). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PPAR gamma/NR1C3 Antibody (NBP1-77833)

Discover more about diseases related to PPAR gamma/NR1C3 Antibody (NBP1-77833).
 

Pathways for PPAR gamma/NR1C3 Antibody (NBP1-77833)

View related products by pathway.

PTMs for PPAR gamma/NR1C3 Antibody (NBP1-77833)

Learn more about PTMs related to PPAR gamma/NR1C3 Antibody (NBP1-77833).
 

Research Areas for PPAR gamma/NR1C3 Antibody (NBP1-77833)

Find related products by research area.

Blogs on PPAR gamma/NR1C3.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PPAR gamma/NR1C3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PPARG
Entrez
Uniprot