Recombinant Human PP2C gamma/PPM1G Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human PP2C gamma/PPM1G Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-546 of Human PP2C gamma/PPM1G

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

Details of Functionality
Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PPM1G
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
61.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 0.1mM PMSF
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human PP2C gamma/PPM1G Protein

  • EC 3.1.3.16
  • MGC1675
  • MGC2870
  • PP2C gamma
  • PP2C, gamma
  • PP2CG
  • PP2CGAMMA
  • PP2C-gamma
  • PPM1C
  • PPM1G
  • PPP2CG
  • Protein phosphatase 1C
  • protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
  • protein phosphatase 1G
  • protein phosphatase 2, catalytic subunit, gamma isoform
  • protein phosphatase 2C gamma isoform
  • Protein phosphatase 2C isoform gamma
  • Protein phosphatase magnesium-dependent 1 gamma
  • protein phosphatase, Mg2+/Mn2+ dependent, 1G

Background

PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that is negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. Recombinant human PPM1G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PPM1G
Entrez