Porcine IgG, Fab2 Native Protein

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA, IHC
Concentration
1.0 mg/ml

Order Details

Porcine IgG, Fab2 Native Protein Summary

Description
Native Porcine IgG, Fab2 protein.

Source: Porcine Serum

Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab')2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Swine IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Swine IgG F(ab')2 molecules lack the Fc portion of Swine IgG and therefore receptors that bind Swine IgG Fc will not bind Swine IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

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