Polyinosinic-polycytidylic acid

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Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Func, In vitro, In vivo, LA
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

Polyinosinic-polycytidylic acid Summary

Immunogen
Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA) that is produced during the replicative life cycle of a majority of viruses. Poly(I:C) is known to induce type-1 interferon (IFN) through TLR3. Poly(I:C) directly binds to TLR3.

Applications/Dilutions

Application Notes
Used for activation of TLR3. Stimulation of TLR3 can be achieved with 10-100 ug/ml. Use in In vitro assay reported in scientific literature (PMID 24743304).Use in Ligand activation, and functional reported in scientific literature (PMID 25957979). Use in In vivo assay reported in scientific literature (PMID 26196739). Use in Ligand Activation reported in scientific literature (PMID 26503951)
Publications
Read Publications using
NBP2-25288 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24693944) Mouse reactivity reported in scientific literature (PMID: 25647836). Rat reactivity reported in scientific literature (PMID: 26603372)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile low endotoxin PBS (<0.05 EU/mg)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

Contents: 1 mg size in 100ul of sterile low endotoxin PBS (<0.05 EU/mg). 10 mg size in 1 ml of sterile low endotoxin PBS (<0.05 EU/mg). 50 mg size - 5 vials of 10mgs in 1 ml sterile low endotoxin PBS (<0.05 EU/mg).

Alternate Names for Polyinosinic-polycytidylic acid

  • Poly I Poly C
  • Poly(I).Poly(C)
  • Poly(I).Poly(C), TLR3 ligand
  • Poly(I:C)
  • Polyinosinic-polycytidylic acid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for Polyinosinic-polycytidylic acid (NBP2-25288)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Func, In vitro, In vivo, LA, WB.


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Func
(4)
In vitro
(9)
In vivo
(4)
LA
(2)
WB
(1)
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Human
(6)
Mouse
(9)
Rat
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP2-25288 Applications Species
Jin M, Komine M, Tsuda H, et al. dsRNA induces IL-33 promoter activity through TLR3-EGFR-IRF3 pathway in normal human epidermal keratinocytes J. Dermatol. Sci. Dec 1 2019 [PMID: 31810599] (Human) Human
Dongre A, Surampudi L, Lawlor RG. Non-canonical Notch signaling drives activation and differentiation of peripheral CD4+ T cells. Name: Frontiers in immunology. 2014 Jan 30 (In vivo, Mouse)

Details:
Mice were injected with 12-15 ug/g body weight of Poly I: Poly C every other day for 5 days.
In vivo Mouse
Sun L, Hult EM, Cornell TT et al. Loss of myeloid-specific protein phosphatase 2A enhances lung injury and fibrosis and results in IL-10 dependent sensitization of epithelial cell apoptosis Am. J. Physiol. Lung Cell Mol. Physiol. Mar 6 2019 [PMID: 30838865] (Func, Mouse) Func Mouse
Kang Y, Nii T, Isobe N, Yoshimura Y. Effects of TLR Ligands on the Expression of Cytokines and Possible Role of NFkB in its Process in the Theca of Chicken Follicles J. Poult. Sci. Apr 24 2018 (Func) Func
Yabe-Wada T, Matsuba S, Takeda K et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci Rep. May 25 2016 [PMID: 27220277] (Func, In vitro, Human, Mouse) Func, In vitro Human, Mouse
Jeong SY, Jeon R, Choi YK et al. Activation of microglial TLR3 promotes neuronal survival against cerebral ischemia. J. Neurochem. 2015 Nov 25 [PMID: 26603372] (In vivo, In vitro, Rat)

Details:
Polyinosinic-polycytidylic acid (Poly-IC or Poly-ICLC ) was used for in vivo experiments in Rats at 1 mg/kg dose - Poly IC injected intraperitoneally after ischemia, while the vehicle group was injected i.p. with 0.9% saline. Poly-IC was used for in vitro experiments also - microglia were treated with vehicle or 1-25 ug/ml of poly-IC.
In vivo, In vitro Rat
Wongchana W, Lawlor RG, Osborne BA, Palaga T. Impact of Notch1 Deletion in Macrophages on Proinflammatory Cytokine Production and the Outcome of Experimental Autoimmune Encephalomyelitis. J. Immunol. 2015 Oct 26 [PMID: 26503951] (In vivo, LA, Mouse)

Details:
Polyinosinic-polycytidylic acid (Poly I:C) was used at 12-15ug/kg body weight in experiments involving generation of Notch1 knockout (N1KO) and CSL/RBP-Jk KOmice from Notch1fl/fl × Mx1 cre+/? (N1KO), Notch1fl/fl × Mx1 cre?/? mice (control), Rbp-jkfl/fl × Mx1 cre+/? mice (CSL/RBP-Jk KO), and Rbp-jkfl/fl X Mx1 cre?/? mice (control).
In vivo, LA Mouse
Beiting DP, Hidano S, Baggs JE et al. The Orphan Nuclear Receptor TLX Is an Enhancer of STAT1-Mediated Transcription and Immunity to Toxoplasma gondii PLoS Biol. 2015 Jul 01 [PMID: 26196739] (In vivo, Mouse)

Details:
Polyinosinic-polycytidylic acid /Poly(I:C) [Imgenex: IMG-2203] was used for in-vivo pre-treatment of Mx1-cre TLXf/f and TLXf/f control mice that were further exposed to infection of Toxoplasma gondii (protozoan parasite). Poly IC was administered intraperitoneally every 3 days, five administrations total of 200 ug/mouse and the data of subsequent experiments may be seen in Figure 7.
In vivo Mouse
Rodriguez DA, Weinlich R, Brown S et al. Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis. Cell Death Differ Jan 1 2016 [PMID: 26024392] (WB, Mouse) WB Mouse
Nohmi K, Tokuhara D, Tachibana D et al. Zymosan Induces Immune Responses Comparable with Those of Adults in Monocytes, Dendritic Cells, and Monocyte-Derived Dendritic Cells from Cord Blood. J. Pediatr. 2015 May 06 [PMID: 25957979] (LA, Func, In vitro, Human)

Details:
Polyinosinic:polycytidylic acid / poly(I:C), TLR3 ligand (Imgenex IMG-2203-10) was used for in-vitro stimulation experiments involving human heparinized cord or adult blood Monocytes, peripheral blood dendritic cells (DCs) and monocyte-derived DCs (MoDCs). poly(I:C) was employed at 50 ug/mL concentration on Monocytes as well as on DCs and at 25 ug/mL on MoDCs. See full text for experimental details and results.
LA, Func, In vitro Human
Show All 19 Publications.

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