Poly-Ubiquitin Antibody (HWA4C4)

Images

 
Western Blot: Poly-Ubiquitin Antibody (HWA4C4) [NBP2-89085] - Western blot of HeLa S100 Fraction (5ug) probed with MAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (Lane A) and MAb to Polyubiquitinylated Conjugates ...read more
Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) [NBP2-89085] - Formalin-fixed paraffin-embedded sections of hippocampus from human Alzheimer's disease brain stained with MAb to Polyubiquitin ...read more
Western Blot: Poly-Ubiquitin Antibody (HWA4C4) [NBP2-89085] - Western blot of single isopeptide-linkage polyubiquitinylated conjugates K6, K11, K27, K29, K33, K48, K63, 1ug each, using MAb to Polyubiquitinylated ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
HWA4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Poly-Ubiquitin Antibody (HWA4C4) Summary

Immunogen
Synthetic peptide containing the ubiquitin K63-isopeptide motif.
Specificity
Recognizes K 63-linked polyubiquitin in wide range of species.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
N/A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Western Blot: Specificity for K63 of mAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) is temperature sensitive. Western blot membranes must not be autoclaved. Excessive heat should be avoided in all applications. K63-linked polyubiquitin modified proteins comprise only a proportion of total cellular polyubiquitinylated species. Increased sample loading may therefore be required for analysis by Western blot. Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
N/A

Alternate Names for Poly-Ubiquitin Antibody (HWA4C4)

  • CEP80
  • HEL112
  • PolyUbiquitin
  • Poly-Ubiquitin
  • ribosomal protein S27a
  • RPS27A
  • S27A
  • UBA80
  • UBC
  • UBCEP1
  • UBCEP80

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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