Podoplanin Antibody

Images

 
Western Blot: Podoplanin Antibody [NBP1-37776] (1ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Podoplanin Antibody Summary

Immunogen
Peptide with sequence C-EKVDGDTQTTVEKD corresponding to internal region according to NP_006465.3, NP_938203.2, NP_001006625.1, NP_001006626.1.
Epitope
internal region
Marker
Lymphatic Endothelium Marker
Specificity
This antibody is expected to recognize all isoforms (NP_006465.3; NP_938203.2; NP_001006625.1; NP_001006626.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDPN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Experiments gave bands at approx. 25 kDa band and 37 kDa band in human brain is reported in scientific literature (PMID: 12032185). This protein has a calculated MW of 24.9 kDa band according to NP_006465.3.
Publications
Read Publications using NBP1-37776.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Podoplanin Antibody

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podoplanin Antibody (NBP1-37776)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Podoplanin Antibody (NBP1-37776). (Showing 1 - 1 of 1 FAQs).

  1. What evidence do you have that it will work in canine tissue?
    • It is made to a portion of the human sequence (listed on the datasheet), and the canine validation is based on sequence homology. We have not directly tested this on canine ourselves.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Podoplanin Antibody (NBP1-37776)

Discover related pathways, diseases and genes to Podoplanin Antibody (NBP1-37776). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Podoplanin.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Bioinformatics

Gene Symbol PDPN
Entrez
Uniprot