Podoplanin Antibody (4D5aE5E6)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-Fr, IHC-P
Clone
4D5aE5E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Podoplanin Antibody (4D5aE5E6) Summary

Immunogen
ORF Human podoplanin ectodomain expressed in E.coli
Marker
Lymphatic Endothelium Marker
Specificity
NB100-65166 recognizes Human Podoplanin (gp36), a 36kDa type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver. Podoplanin is absent on blood endothelium and therefore NB100-65166 is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumour lymphangiogenesis.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen .75 ug/ml-2 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Podoplanin Antibody (4D5aE5E6)

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Podoplanin Antibody (NB100-65166)

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Research Areas for Podoplanin Antibody (NB100-65166)

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Blogs on Podoplanin.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Bioinformatics

Gene Symbol PDPN
Entrez
Uniprot