Podocalyxin Like Antibody


Western Blot: Podocalyxin Antibody [NB110-41510] - Detection of podocalyxin in human lung lysate.
Immunocytochemistry/ Immunofluorescence: Podocalyxin Antibody [NB110-41510] - Podolyxin antibody was tested in V6.5 MES cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
1.06 mg/ml

Podocalyxin Like Antibody Summary

A synthetic peptide within an internal region (residues 350-450) of the human Podocalyxin protein. [Swiss-Prot# O00592]
Podocytes /Apical Marker, Endothelial Cell Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1.06 mg/ml
Immunogen affinity purified


  • Western Blot 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This Podocalyxin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In WB a band is observed at ~52 kDa. There may be a non-specific band observed at ~27 kDa depending on the lysates used. In ICC/IF membrane staining was observed.

Reactivity Notes

Human and mouse. Immunogen sequence has 80% homology to canine and 76% homology to rat.

Alternate Names for Podocalyxin Like Antibody

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin-like protein 1
  • podocalyxin-like


Podocalyxin (PODXL) is an extensively N/O-glycosylated and sialylated type I transmembrane protein that contains C-terminal PDZ-binding motif DTHL capable of facilitating interactions with NHERF1/NHERF2 adaptor proteins which complexes with proteins implicated in ion transport, protein trafficking and signal transduction. PODXL function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in podocytes by charge repulsion. PODXL also acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. It induces apical actin-dependent microvilli formation and is implicated preapical plasma membrane subdomain formation to set up inital epithelial polarization and apical lumen formation during renal tubulogenesis. PODXL is highly expressed in renal glomeruli, embryonic germ layers, hematopoietic progenitors, megakaryocytes and platelets, vascular endothelia, mesothelial cells lining organs, and neurons. In several malignancies such as leukemia and cancer of breast, testicular, prostate etc, PODXL interactions with actin-binding protein EZR enhances cancer aggressiveness by inducing cell migration and invasion through via modulation of MAPK/PI3K signaling.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Podocalyxin Like Antibody (NB110-41510): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Podocalyxin Like Antibody (NB110-41510) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-41510AF405
Alexa Fluor (R) 488 Labeled NB110-41510AF488
Alexa Fluor (R) 647 Labeled NB110-41510AF647
Alexa Fluor (R) 700 Labeled NB110-41510AF700
Biotin Labeled NB110-41510B
DyLight 405 Labeled NB110-41510V
DyLight 488 Labeled NB110-41510G
DyLight 550 Labeled NB110-41510R
DyLight 650 Labeled NB110-41510C
FITC Labeled NB110-41510F
HRP Labeled NB110-41510H

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Contact Information

Product PDFs


Gene Symbol PODXL

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