Podocalyxin Like Antibody


Western Blot: Podocalyxin Antibody [NB110-41510] - Detection of podocalyxin in human lung lysate.
Immunocytochemistry/ Immunofluorescence: Podocalyxin Antibody [NB110-41510] - Podolyxin antibody was tested in V6.5 MES cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more

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Podocalyxin Like Antibody Summary

A synthetic peptide within an internal region (residues 350-450) of the human Podocalyxin protein. [Swiss-Prot# O00592]
Podocytes /Apical Marker, Endothelial Cell Marker
Immunogen affinity purified
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  • Western Blot 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This Podocalyxin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In WB a band is observed at ~52 kDa. There may be a non-specific band observed at ~27 kDa depending on the lysates used. In ICC/IF membrane staining was observed.
Positive Control
Lung Lysate (NB820-59235)
Podocalyxin Like Lysate (NBP2-64920)

Reactivity Notes

Human and mouse. Immunogen sequence has 80% homology to canine and 76% homology to rat.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.06 mg/ml
Immunogen affinity purified

Alternate Names for Podocalyxin Like Antibody

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like


Podocalyxin (PODXL) is an extensively N/O-glycosylated and sialylated type I transmembrane protein that contains C-terminal PDZ-binding motif DTHL capable of facilitating interactions with NHERF1/NHERF2 adaptor proteins which complexes with proteins implicated in ion transport, protein trafficking and signal transduction. PODXL function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in podocytes by charge repulsion. PODXL also acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. It induces apical actin-dependent microvilli formation and is implicated preapical plasma membrane subdomain formation to set up inital epithelial polarization and apical lumen formation during renal tubulogenesis. PODXL is highly expressed in renal glomeruli, embryonic germ layers, hematopoietic progenitors, megakaryocytes and platelets, vascular endothelia, mesothelial cells lining organs, and neurons. In several malignancies such as leukemia and cancer of breast, testicular, prostate etc, PODXL interactions with actin-binding protein EZR enhances cancer aggressiveness by inducing cell migration and invasion through via modulation of MAPK/PI3K signaling.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol PODXL