PNPO Antibody (2F8)

Images

 
Western Blot: PNPO Antibody (2F8) [NBP2-00961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: PNPO Antibody (2F8) [NBP2-00961] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PNPO.
Flow Cytometry: PNPO Antibody (2F8) [NBP2-00961] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNPO antibody, and then analyzed by ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00961
    • Availability
      Product Discontinued

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PNPO Antibody (2F8) Summary

Immunogen
Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PNPO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PNPO Antibody (2F8)

  • EC 1.4.3.5
  • FLJ10535
  • PDXPO
  • pyridoxal 5'-phosphate synthase
  • pyridoxamine 5'-phosphate oxidase
  • Pyridoxamine-phosphate oxidase
  • pyridoxine 5'-phosphate oxidase
  • pyridoxine-5'-phosphate oxidase

Background

The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PNPO
Entrez