PNMT Antibody (2D5)

Images

 
Western Blot: PNMT Antibody (2D5) [NBP2-00692] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: PNMT Antibody (2D5) [NBP2-00692] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT.
Immunohistochemistry-Paraffin: PNMT Antibody (2D5) [NBP2-00692] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody.
Flow Cytometry: PNMT Antibody (2D5) [NBP2-00692] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNMT antibody, and then analyzed by ...read more
Immunocytochemistry/ Immunofluorescence: PNMT Antibody (2D5) [NBP2-00692] - Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody.
Flow Cytometry: PNMT Antibody (2D5) [NBP2-00692] - Analysis of Hela cells, using anti-PNMT antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PNMT Antibody (2D5) [NBP2-00692] - Analysis of Jurkat cells, using anti-PNMT antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00692
    • Availability
      Product Discontinued

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PNMT Antibody (2D5) Summary

Immunogen
Full length human recombinant protein of human PNMT (NP_002677) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PNMT
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PNMT Antibody (2D5)

  • EC 2.1.1
  • EC 2.1.1.28
  • Noradrenaline N-methyltransferase
  • PENT
  • PENTMGC34570
  • phenylethanolamine N-methylase
  • phenylethanolamine N-methyltransferase
  • PNMT
  • PNMTase

Background

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PNMT
Entrez