PMEL17/SILV Antibody (9G3)

Images

 
Western Blot: PMEL17/SILV Antibody (9G3) [NBP1-47971] Analysis of extracts (35ug) from 9 different cell lines by using anti-PMEL17/SILVmonoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody (9G3) [NBP1-47971] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (9G3) [NBP1-47971] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SILV mouse monoclonal antibody.
Western Blot: PMEL17/SILV Antibody (9G3) [NBP1-47971] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (9G3) [NBP1-47971] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (9G3) [NBP1-47971] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (9G3) [NBP1-47971] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SILV mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47971
    • Availability
      Product Discontinued

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PMEL17/SILV Antibody (9G3) Summary

Immunogen
Protein expressed in 293T cell transfected with human SILV expression vector
Marker
Melanosome Marker
Specificity
This antibody is specific for Homo sapiens silver homolog (mouse) (SILV).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
70.225 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PMEL17/SILV Antibody (9G3)

  • D12S53EP1
  • gp100
  • ME20
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Background

Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMEL17/SILV Antibody (NBP1-47971). (Showing 1 - 1 of 1 FAQ).

  1. Do you happen to know what the specific epitope of this antibody is?
    • We unfortunately do not epitope map our products, so I am not certain what epitope this antibody will bind to. The immunogen is protein expressed in 293T cell transfected with human SILV expression vector. The full length protein sequence was used, UniProt P40967.

Secondary Antibodies

 

Isotype Controls

Additional PMEL17/SILV Products

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Bioinformatics

Gene Symbol PMEL
Entrez