PMEL17/SILV Antibody (7E3)

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Flow Cytometry: PMEL17/SILV Antibody (7E3) [NBP1-47975] HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PMEL17/SILV antibody, and then ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47975
    • Availability
      Product Discontinued

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PMEL17/SILV Antibody (7E3) Summary

Immunogen
Protein expressed in 293T cell transfected with human PMEL17/SILV expression vector ((NP_008859)
Marker
Melanosome Marker
Specificity
This antibody is specific for Homo sapiens silver homolog (mouse) PMEL17/SILV.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PMEL17/SILV Antibody (7E3)

  • D12S53EP1
  • gp100
  • ME20
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Background

Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for PMEL17/SILV Antibody (NBP1-47975). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know if you have any more info on Melanoma gp100 Antibody (NBP1-47975). I need to sort human melanoma cells by FACS and I would like to know the quality of this antibody. Do you have anything already conjugated?
    • Our lab used the following protocol for the testing of NBP1-47975 in FACS: Flow Cytometry for Intracellular Staining Solutions and Reagents 1X Phosphate Buffered Saline (PBS): Dissolve 8g NaCl, 0.2g KCl, 1.15g Na2HPO4 and 0.2g KH2PO4 in 800mL distilled water (dH2O). Adjust the pH to 7.4 with HCl and the volume to 1 liter. Store at room temperature. Fixation buffer: 2% paraformaldehyde in 1xPBS Permeabilization buffer : 0.1% Triton X-100 in 1xPBS FACS buffer: 0.5% BSA, 0.05% Azide in 1xPBS Fluorescent dye conjugated secondary antibody. Fixation Collect cells by centrifugation and aspirate supernatant. Fix the cell by 125ul cold fixation buffer, vortex briefly. Incubate at room temperature for at least 30 min or for 1hr 40C. Centrifuge for 5min at 300g,remove the supernatant. Permeabilization Add 1ml permeabilization buffer to each tube. Centrifuge briefly, and aspirate supernatant. Resuspend cells in 125ul of permeabilization buffer and incubate at room temperature for 5min. Staining Aliquot 1-2x106 cells into each tube. Add 1 ml FACS buffer to each tube, centrifuge to pellet the cells. Resuspend cell pellet with 125ul FACS buffer containing diluted primary antibody, vortex and incubate on ice for 30min. Rinse as before in FACS buffer by centrifugation. Resuspend cells in fluorescent dye conjugated secondary antibody, diluted in FACS buffer per manufacturer's recommendations. Incubate for 30 minutes on ice. Rinse the cells as before in FACS Buffer by centrifugation. Resuspend cells in 0.5 ml PBS and analyze on flow cytometer. We do not have a conjugated form of this antibody currently available, but we can do a custom conjugation for an additional fee. I recommend using a conjugated secondary with this antibody if it's possible for your assay. All of our products are covered by a 100% guarantee for the applications and species listed on the data sheets. If one of our antibodies does not perform as promised, then we would be happy to replace it or provide a refund.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PMEL
Entrez
Uniprot