PLK1 [p Thr210] Antibody

Images

 
Western Blot: PLK1 [p Thr210] Antibody [NB100-78394] - Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK monoclonal ...read more
Immunocytochemistry/ Immunofluorescence: PLK1 [p Thr210] Antibody [NB100-78394] - Immunofluorescent microscope analysis of nocodazole treated Hela cells (10iM for overnight), using PLK-1 Phosphorylated (Thr210) ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78394
    • Availability
      Product Discontinued

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PLK1 [p Thr210] Antibody Summary

Immunogen
Modified peptide
Modification
p Thr210
Marker
Mitosis Marker
Specificity
PLK-1 [Thr210]
Clonality
Polyclonal
Host
Rabbit
Gene
PLK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
PLK1 Lysate (NBL1-14515)
Publications
Read Publications using
NB100-78394 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PLK1 [p Thr210] Antibody

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The Poly6186 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. The Poly6186 antibody cross-reacts with an unknown kinase in non-mitotic cells. To increase specificity, it is recommended that the Poly6186 antibody be used for Western blotting after immunoprecipitation with a pan-specific PLK-1 antibody (clone 3F8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLK1 Antibody (NB100-78394)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLK1
Entrez
Uniprot