Western Blot: PLK1 [p Thr210] Antibody [NB100-78394] - Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK monoclonal ...read more
Immunocytochemistry/ Immunofluorescence: PLK1 [p Thr210] Antibody [NB100-78394] - Immunofluorescent microscope analysis of nocodazole treated Hela cells (10iM for overnight), using PLK-1 Phosphorylated (Thr210) ...read more
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Read Publications using NB100-78394 in the following applications:
PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The Poly6186 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. The Poly6186 antibody cross-reacts with an unknown kinase in non-mitotic cells. To increase specificity, it is recommended that the Poly6186 antibody be used for Western blotting after immunoprecipitation with a pan-specific PLK-1 antibody (clone 3F8).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.